The Puppini Sisters are a musical trio specializing in 1940s-style close harmony vocal music.

The members are Italian Marcella Puppini and English Stephanie O'Brien and Kate Mullins. Although the three are not related, they chose their name in tribute to The Andrews Sisters. Puppini first studied fashion design at St. Martins School of Art, and later joined Stephanie O'Brien and Kate Mullins at Trinity College of Music in London. The Puppini Sisters are currently supported by a three-piece band featuring Martin Kolarides on guitar, Henry Tyler on drums/percussion and upright bassist Henrik Jensen.

The group was founded by Puppini after she was inspired by the animated film Belleville Rendez-vous. The Puppini Sisters' debut single, "Boogie Woogie Bugle Boy", is a cover of the iconic hit single by The Andrews Sisters. One of the more unusual songs in their repertoire is Kate Bush's "Wuthering Heights".

The Puppini Sisters' second album, The Rise and Fall of Ruby Woo, followed in the tongue-in-cheek footsteps of Betcha Bottom Dollar, and included original compositions by Puppini, O'Brien and Mullins. The Ruby Woo of the album title is a reference to MAC Cosmetics' 'Ruby Woo' lipstick: a favourite of the rockabilly scene and of the Puppini Sisters themselves. 'Ruby Woo' is also the preferred lipstick of The Cure frontman Robert Smith, a singer Marcella Puppini has admitted she would love to duet with.

The Puppini Sisters were originally scheduled to appear on Making Your Mind Up, the United Kingdom's preselection show for the Eurovision Song Contest 2007, however their submitted material was deemed inappropriate.